BioCoR faculty are committed to providing review articles on relevant topics in preservtion. The following is a listing of recent review aricles by BioCoR faculty that you may find helpful.
Hubel A, Aksan A, Skubiz APN, Wendt C and Zhong X. "State of the art in preservation of fluid biospecimens", Biopreservation & Biobanking, 9:237-244, 2011.
A review of factors that influence quality of frozen biospecimens including plasma and serum, urine, saliva, cerebrospinal fluid, and bronchoalveolar lavage.
Unhale S, Solomon R, Skubitz APN, Hubel A. "Stabilization of tissue specimens for pathological examination and biomedical research", Biopreservation & Biobanking, 10(6): 493-500, 2012.
This review describes factors that influence the quality of tissue biospecimens that are either frozen or chemically fixed.
Preservation of cell therapies
Hanna J and Hubel A. "Preservation of stem cells", Organogenisis, 5:134-137, 2009.
This review describes recent advantages in the preservation of hematopietic stem cells, mesenchymal stem cells and human embryonic stem cells.
Hubel A. "Advancing the preservatio of cellular therapy products", Transfusion, 51(S1, Supplement 4): 82S*86S, 2011.
This review discusses emerging issues in preservation that are critical for improving preservation of cell therapies.March 13, 2016
ASME Journal of Biomechanical Engineering Biotransport Special Issue co-edited by BioCoR faculty is published. This special issue focuses on current and evolving areas of emphasis in biotransport, including important applications of biopreservation (molecular, cellular and tissue) and thermal therapies (high temperature - laser, radiofrequency, microwave, high intensity ultrasound heating of cells and tissues, and low temperature - cryosurgery). The contributions, which include reviews and original work, are in four main areas: thermodynamics, multi-scale mass transfer, multi-scale heat transfer and extreme biology.
See the table of contents of this special issue on the ASME Digital Library at:October 14, 2009
Khuu, H.M., et al., Catastrophic failures of freezing bags of cellular therapy products: description, case, and consequences. Cytotherapy, 2002. 4(6): p. 539-49.
The article describes the catastrophic failure of bags used to cryopreserve cell therapy products. Why we like it: Selection of proper cryopreservation container is critical as you are storing your biospecimens at very low temperature and failure of the container is typically catastrophic. This article compares different freezing containiers. In addition, this study describes the process used to determine whether or not a problem existed and the analysis used to confirm that there was indeed a problem.February 8, 2010
BioCoR faculty manuscript on confocal Raman microspectroscopy of frozen protein solutions was presented in J. Phys. Chem. B main page http://pubs.acs.org/journal/jpcbfk.
For full article visit http://pubs.acs.org/doi/abs/10.1021/jp809710d.July 2, 2009
BioCoR featured in Clinical Laboratory NewsMarch 13, 2016
BioLifeSolutions signed a license and custom cGMP manufacturing agreement with Centocor R&D. The company will produce a variant of its serum-free and protien-free CryoStor biopreservation media product, which is formulated with a reduced concentration of 2% DMSO. BioLife specializes in the deveopment of patented hypothermic storage/transport and cryopreservation media products for cells, tissues, and organs. The company claims its GMP products are serum/protien-free and preformulated to reduce delayed-onset cell damage and death. In November BioLife was granted a Japanese patent covering claims related to protecting cells from injury and death caused by cold temperatures used in biopreservation. BioLife was previously granted equivalent U.S. and European patent protection for the IP. In September Sigma Aldrich negotiated a nonexclusive distribution agreement for BioLife's HyopThermosol and CryoStor products.December 18, 2009
Kisland K, Kerna I, Kumm J, Jonsson, H, Tamm A, "Impact of cryopreservation on serum concentration of metalloptoteinases (MMP)-7, TIMP-1, vascular endothelial growth factor (VEGF) and VEGF-R2 in Biobank samples," Clin Chem Lab Med 49 (2): 229-235, 2011.
Why it is important: Studies on the stability of these biomarkers have addressed issues of collection process but not storage process on stability of the markers. The study also used accelerated aging to determine stability of markers with storage. Certain markers were very sensitive to repeated freeze-thaw cycles (VEGF-R2). Stability with storage was also determined and TIMP-1 and VEGF demonstrated very poor stability with storage at ~-80oC.
March 28, 2011
Nietfield JJ, Sugarman J, Litton JE, "The Bio-PIN: a concept to improve biobanking," Nature Reviews Cancer, 11: 303-308, 2011.
Why it is important: The paper proposes the structures of a method of identifying biospecimens based on single nucleotide polymorphisms. Issues of identification of samples and tracking of consent are complex. This article presents an interesting option for dealing with these issues.
March 28, 2011
Citation: "Recommendations for Cell Banks Used in GXP Assays, Preparation, Characterization, and Storage" Ana T. Menendez, Nadien Ritter, Jonathan Zmuda, Darshana Jani, and Jaya Goyal, BioProcess International, 10(1): 28-40, 2012.
Why it is important: It is a well written, logically laid out and discusses a wide range of issues. Training of personnel, calibration and maintenance of equipment, documentation, a flow chart of the process, and a discussion of risk managment are all discussed. The only concern we have is that cryovials are still being used. There are sterile, closed vials available and for both master and working cell banks, closed systems should be used.August 18, 2011
Citation: Chakraborty N, Menze MA, Malsam J, Aksan A, Hand SC, et al. 2011 Cryopreservation of Spin-Dried Mammalian Cells. PLoS ONE 6(9): e24916. doi:10.1371/journal.pone.0024916
Why it is important: This study reports an alternative approach to achieve vitrification where cells are pre-desiccated prior to cooling to cryogenic temperatures for storage. Chinese Hamster Ovary (CHO) cells suspended in trehalose solution were rapidly and uniformly desiccated to a low moisture content (<0.12 g of water per g of dry weight) using a spin-drying technique. Trehalose was also introduced into the cells of using a high-capacity trehalose transporter (TRET1). Fourier Transform Infrared Spectroscpy (FTIR) was used to examine the uniformity of water concentration distribution in the spin-dried samples. 62% of the cells were shown to survive spin-drying in the presence of trehalose following immediate rehydration. The spin-dried samples were stored in liquid nitrogen (LN2) at a vitrified state. It was shown that following re-warming to room temperature and re-hydration with a fully complemented cell culture medium, 51% of the spin-dried and vitrified cells survived and demonstrated normal growth characteristics. Spin-drying is a novel strategy that can be used to improve cryopreservation outcome by promoting rapid vitrification.March 13, 2016
Mason C, Brindley DA, Culme-Seymour EJ, Davie NL, "Cell therapy Industry: billion dollar global buisness with unlimited potential", Regen Med 6(3); 265-272, 2011.
Why it is important: It demonstrates the manner by which tissue engineering/regenerative medicine has been eclipsed by cell therapy and the very real economic impact of cell therapy today.
August 18, 2011
Cao et al. Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions. Biotechnol. Bioeng. (2003) vol. 82 (6) pp. 684-90
The article clearly describes the importance of both cooling and thawing rates on the post thaw activity of proteins. Why we like it: it debunks the myth that protein solutions are not influences by the method/rate of cooling or thawing. The study is well done and rigorous.June 23, 2010
Effects of Storage Time and Exogenous Protease Inhibitors on Plasma Protein Levels Ayache et. al. american Journal of Clinical Pathology, 2006 vol. 126 (2) pp. 174-184.
This is an elegant study demonstrating the significant changes in a wide variety (~37) of biomarkers with a 2-hour hold prior to processing. The study also evaluates the influence of protease inhibitors sample behavior. Why we like this article: Beyond the issues of handling and short-term storage of plasma, the study provoked an excited discussion amongst BioCoR facutly who could see the implications of the study extending far beyond the conclusions made in the paper.January 26, 2010
Efforts to improve vaccine stabilization heat up. When researchers tracked the temperature of hepatitis B vaccine being delivered to frigid, remote reaches of western China in 2007, they found vaccine vials spending a median time of more than four days below their freezing point of minus 0.5 degrees Celsius.
Unlike other types of blood components, refrigeration of platelets leads to their rapid clearance from the circulation after transfusion. Platelets must therefore be stored at room temperature, a serious limitation to their use for transfusions. Viktoria Rumjantseva et al. now dissect two platelet clearance pathways by which exposed carbohydrate residues on platelets are recognized by receptors on liver macrophages and hepatocytes, which differentially control the clearance of short-term- and long-term- refrigerated platelets.
Despite progress in the biosensor field, a platform that allows the sensitive detection of disease-specific proteins in a diverse range of clinical samples such as saliva, serum and urine has proved elusive. Here, Richard Gaster and his colleagues introduce a magnetic nanosensing protein detection platform that offers quantitative multiplex protein detection at attomolar concentrations over a large linear dynamic range and in a range of biological fluids.
November 6, 2009
Freezing of Tissue Specimens: bibliography for Tissue Freezing TechniquesAugust 18, 2011
GenomeWeb Feature: Amid Array of Biobanking Obstacles, Researchers Work on Solutions
By Matt Jones
New York (GenomeWeb News) - When the biomedical research community was still abuzz over the completion of the Human Genome Project, three scholars at the Massachusetts Institute of Technology published a sobering article. Although biobanks could be great enablers of genome-based personalized medicine, they said, such repositories are fraught with problems.December 18, 2009